ADVANCES IN BIOMEDICAL OPTICS SEMINAR: "High-resolution Imaging of Cerebral Oxygen Metabolism and Hemodynamic Responses"

Thu, 12/01/2016 - 12:00
Sava Sakadzic (Harvard)

The understanding of the cortical oxygen delivery and consumption on the microvascular scales may have profound implications for evaluating microvascular oxygen delivery capacity to support cerebral tissue metabolism in health and disease, and for quantitative interpretation of signals in macroscopic imaging modalities such as BOLD fMRI. To address these challenges, we developed a set of optical microscopy technologies for in vivo measurement of cerebral hemodynamic and metabolic parameters at subcapillary scales, including a first practical two-photon microscopy imaging of oxygen partial pressure in cortical tissue and microvasculature. We used these tools in combination with numerical modeling to quantify oxygen extraction from various microvascular segments at rest, to develop novel method for measuring CMRO2 based on periarteriolar PO2 gradients, to bottom-up model BOLD fMRI signal from oxygen distribution in the microvasculature, and to quantify hemodynamic responses to functional activation in awake and anesthetized animals.


*These seminars are supported by the Biomedical Imaging and Spectroscopy Laboratory, the Center for Magnetic Resonance and Optical  Imaging, the Department of Radiology and the Department of Physics and Astronomy at the University of Pennsylvania.

*Organizers: Wesley Baker, Jeff Cochran, Bryan Chong, Tiffany Ko, and Arjun Yodh,

*Contact: Wesley Baker

Donner Auditorium, Basement, Donner Building, 3400 Spruce St.