Condensed Matter Seminar: "Single-molecule force spectroscopy: Theory meets experiment"

Wed, 09/18/2013 - 16:00 - 17:00
Olga Dudko, U.C. San Diego

Single-molecule biophysical tools are making it possible to measure the response of individual biomolecules to external force – in real time and with unprecedented resolution – revealing information that is typically lost when studied though traditional “bulk” methods. I will present a general analytical theory of force-induced molecular transitions. The theory is based on Kramers picture of diffusive barrier crossing and on a generalization of this picture to many dimensions. The theoretical procedure of interpreting experimental data will be illustrated with (i) unfolding of riboswitches and (ii) unbinding of a receptor-ligand complex involved in blood clot formation with optical tweezers, (iii) unzipping of individual DNA hairpins with nanopores, and (iv) unfolding of proteins with an atomic force microscope.

Room A4, DRL22